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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections
doi: 10.1073/pnas.1219206110
Figure Lengend Snippet: Workflow of the all-optical correlative live-cell and superresolution imaging. A live-cell time-lapse movie is recorded at high temporal resolution. The sample is then fixed in situ and stained with antibodies conjugated with photoswitchable fluorophores for immunofluorescence and superresolution imaging (STORM). A STORM image of the microtubule network is then recorded. Single-particle tracking is used to obtain trajectories from the live-cell movie, and these trajectories are precisely aligned with the STORM image of the microtubules using fiduciary markers. IF, immunofluorescence.
Article Snippet: Lysosomes in live-cell images were tracked with an automated
Techniques: Imaging, In Situ, Staining, Immunofluorescence, Single-particle Tracking